This document is an operation guide for the Th1/Th2 Subgroup Monitoring Test Kit on a single laser Calibur flow cytometer. The guide provides detailed instructions on setting up the machine and acquiring samples.
This document serves as an operation guide for using the Th1/Th2 Subgroup Monitoring Test Kit in conjunction with a Single Laser Calibur flow cytometer. It details the initial machine condition settings required before analysis begins.
The procedure includes specific steps for preparing calibration beads and buffers, adjusting voltage thresholds (FSC, SSC) and fluorescence channels (FL1, FL2, FL3), and setting compensation parameters to ensure accurate data collection. This manual is written in Chinese.
The FSC threshold must be adjusted to a value of 650.
The system should be set to collect approximately 300 beads for each population.
The Resolution value must be adjusted to 1024.