A reference chart listing fluorochrome stain index, brightness, excitation lasers, and instrument compatibility for BD flow cytometers.
The Fluorochrome Reference Chart provides a quick‑reference guide for selecting fluorochromes in multicolor flow cytometry. It includes stain index values, relative brightness rankings, and the laser lines and detector channels used by various BD instruments.
The document also offers practical guidelines for reagent selection, such as minimizing spectral spillover, matching bright fluorochromes to dim antigens, and handling tandem dyes. Links to BD web tools for spectra and color selection are included.
| Stain Index formula | D/W |
|---|---|
| Simultaneous detection capacity | 8 colors (4 blue laser, 2 red laser, 2 violet laser) |
The chart states the system can detect 8 colors simultaneously (4 blue laser, 2 red laser, 2 violet laser).
Stain index is defined as D divided by W, measuring resolution sensitivity between positive and background peaks.
Brilliant Violet™ 421 is listed as the brightest fluorochrome.