Key facts
- Manuals
- 1
- Languages
- EN
- Last added
- 2026-10-01
Featured BD Biosciences manuals
BD Biosciences Fluorochrome Reference Chart
The Fluorochrome Reference Chart provides a quick‑reference guide for selecting fluorochromes in multicolor flow cytometry. It includes stain index values, relative brightness rankings, and the laser lines and detector channels used by various BD instruments.
The document also offers practical guidelines for reagent selection, such as minimizing spectral spillover, matching bright fluorochromes to dim antigens, and handling tandem dyes. Links to BD web tools for spectra and color selection are included.
Covers: Relative Brightness · Instrument Excitation Laser Line · Guidelines for Selecting Reagents for Multicolor Flow Cytometry · The Basics: Know Your Instrument · Fluorochromes: Go for the Bright · Minimize Spillover
Latest BD Biosciences manuals
BD Biosciences Fluorochrome Reference Chart
Product Leaflet 1 pages · 457 KB · ENA reference chart listing fluorochrome stain index, brightness, excitation lasers, and instrument compatibility for BD flow cytometers.
Questions answered in BD Biosciences manuals
How many colors can be detected simultaneously?
The chart states the system can detect 8 colors simultaneously (4 blue laser, 2 red laser, 2 violet laser).
What does the stain index represent?
Stain index is defined as D divided by W, measuring resolution sensitivity between positive and background peaks.