E.coli Competent Cells JM109 is a high-efficiency competent cell produced by Takara and can be used to prepare gene libraries, recombinant plasmids and clone experiments.
This manual describes the TaKaRa JM109 competent cells, a chemically‑competent E.coli strain (genotype recA, endA’, gyrA96, thi1, etc.) supplied as a frozen aliquot for molecular cloning. The product is intended for research use only and is not approved for therapeutic, diagnostic, food, cosmetic, or household applications.
The document outlines the recommended storage temperature (‑80 °C), the composition of the recovery medium, and step‑by‑step transformation procedures using pUC19 plasmid DNA. It also lists typical transformation efficiencies (≈1 × 10⁸ transformants per 1 pg pUC19) and provides safety and handling notes.
| Cell concentration | 1 ~ 2 × 10⁹ cells |
|---|---|
| Transformation efficiency (pUC19) | 1 × 10⁸ transformants per 1 pg DNA |
| Recovery medium – Bacto tryptone | 2 % (w/v) |
| Recovery medium – Bacto yeast extract | 0.5 % (w/v) |
| Recovery medium – NaCl | 10 mM |
| Recovery medium – KCl | 2.5 mM |
| Recovery medium – MgSO₄ | 10 mM |
| Recovery medium – MgCl₂ | 10 mM |
| Recovery medium – Glucose | 20 mM |
Approximately 1 × 10⁸ transformants are obtained per 1 pg of pUC19 plasmid DNA.
No, the product is for research use only and not intended for therapeutic or diagnostic procedures.