E.coli Electro-Cells HST02 is a high efficiency electroporation cell, suitable for methylation DNA transformation. This host can be used as M13 phage vector DNA host cell to prepare single-stranded DNA, it can also be used to prepare DNA library or clone.
The Electro‑Cells HST02 kit contains ten 50 µL electro‑competent cells and a control pUC19 DNA (0.01 ng) tube. Cells have a concentration of 1‑2 × 10¹⁰ cells · mL⁻¹ and should be stored at –80 °C until use. The kit is designed for high‑efficiency transformation of plasmid DNA and for preparation of M13 phage DNA.
To use, thaw the Electro‑Cell on ice, add 1‑2 µL DNA (max 10 ng per 50 µL), and electroporate at 15 kV. After electroporation, add 1 mL SOC medium and incubate at 37 °C with shaking (160‑225 rpm) for 1 hour before plating on selective agar. The manual also details M13 phage DNA preparation, storage precautions, and the composition of the SOC medium. This manual is written in Chinese.
| Kit contents | Electro‑Cells HST02 50 µL × 10 tubes; Control DNA (pUC19, 0.01 ng) 10 × 1 tube |
|---|---|
| Cell concentration | 1‑2 × 10¹⁰ cells · mL⁻¹ |
| Maximum DNA per reaction | 10 ng per 50 µL cell suspension |
| SOC medium composition | 2% Bacto tryptone, 0.5% Bacto yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgSO₄, 10 mM MgCl₂, 20 mM Glucose |
Store the Electro‑Cell at –80 °C and do not refreeze after thawing.
Do not exceed 10 ng of DNA per 50 µL cell suspension.
SOC contains 2% Bacto tryptone, 0.5% Bacto yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgSO₄, 10 mM MgCl₂, and 20 mM glucose.