Key facts
- Manuals
- 12
- Languages
- ZH · JA · EN
- Last added
- 2012-01-21
Featured E.coli manuals
Takara HB101 Electro-Cells User Manual
The Takara HB101 Electro-Cells are high‑efficiency competent E. coli cells designed for gene‑recombination experiments. They contain the genotype SUDE44, ADCR‑An1T, recA413, among others, and are supplied as 50 µL aliquots (10 × HB101) with control DNA (PBR322, 0.01 ng/µL). The cells are stored at –80 °C and must be thawed on ice before use.
The manual describes the electroporation protocol: thaw the Electro‑Cell, add 1–2 µL DNA (desalting if needed), mix in a pre‑chilled 0.1 cm cuvette, apply a high‑voltage pulse, immediately add 1 mL SOC medium, incubate at 37 °C for 1 h with shaking, then plate on agar. Precautions include dry‑ice transport, avoiding refreezing after thaw, and limits on DNA size and amount for optimal efficiency. This manual is written in Chinese.
Covers: Product Overview · Genotype Information · Cell Concentration · Transformation Procedure · Storage Guidelines · Precautions
Takara JM109 Electro-Cells User Manual
The Takara JM109 Electro‑Cells are high‑efficiency competent E. coli cells supplied with 0.01 ng/µL pUC19 DNA as a control. They should be stored at –80 °C and thawed just before use. The manual details the genotype of the JM109 strain and provides guidelines for handling the cells.
The document describes a step‑by‑step electroporation procedure, including cell preparation, DNA addition, electroporation at 15 kV, recovery in SOC medium, and plating on selective agar. Additional protocols for M13 phage DNA preparation and a list of precautions (e.g., using sterile water, avoiding repeated freeze‑thaw cycles) are also included. This manual is written in Japanese.
Covers: Product Overview · Storage Conditions · Genotype Information · Transformation Procedure · M13 Phage DNA Preparation · Precautions and Safety
Takara Electro-Cells HST02 User Manual
The Electro‑Cells HST02 kit contains ten 50 µL electro‑competent cells and a control pUC19 DNA (0.01 ng) tube. Cells have a concentration of 1‑2 × 10¹⁰ cells · mL⁻¹ and should be stored at –80 °C until use. The kit is designed for high‑efficiency transformation of plasmid DNA and for preparation of M13 phage DNA.
To use, thaw the Electro‑Cell on ice, add 1‑2 µL DNA (max 10 ng per 50 µL), and electroporate at 15 kV. After electroporation, add 1 mL SOC medium and incubate at 37 °C with shaking (160‑225 rpm) for 1 hour before plating on selective agar. The manual also details M13 phage DNA preparation, storage precautions, and the composition of the SOC medium. This manual is written in Chinese.
Covers: Product Description · Storage Conditions · Genotype Information · Transformation Procedure · M13 Phage DNA Preparation · Precautions
Takara BMH71-18 Competent Cells User Manual
The BMH71-18 competent cells are a Takara‑produced E.coli strain designed for high‑efficiency transformation of low‑quantity DNA, such as plasmid or phage DNA. The cells are supplied lyophilized and should be stored at –80 °C until use.
The manual details the thaw‑and‑transform procedure, including the recommended DNA amount (≤10 ng), incubation steps at 42 °C and 37 °C, and the composition of SOC recovery medium. Precautions such as using sterile water and avoiding repeated freeze‑thaw cycles are also provided. This manual is written in Japanese.
Covers: Product Overview · Genotype Information · Transformation Procedure · Precautions · SOC Medium Composition
Latest E.coli manuals
Takara HB101 Electro-Cells User Manual
User Manual 1 pages · 233 KB · ZHInstructions for using Takara HB101 Electro-Cells to transform E. coli by electroporation.
Takara JM109 Electro-Cells User Manual
User Manual 1 pages · 198 KB · JAInstructions for using Takara JM109 electro‑competent cells, covering storage, transformation protocol, M13 phage preparation and safety notes.
Takara Electro-Cells HST02 User Manual
User Manual 1 pages · 243 KB · ZHThis manual provides step‑by‑step instructions for using Takara Electro‑Cells HST02 for DNA transformation and M13 phage preparation.
E.coli HST08 Premium Electro-Cells Manual
E.coli Electro-Cells DH5α Manual
E.coli Competent Cells HB101 Manual
E.coli Competent Cells MV1184 Manual
Takara BMH71-18 Competent Cells User Manual
User Manual 1 pages · 249 KB · JAInstructions for using Takara BMH71-18 E.coli competent cells, covering storage, transformation protocol, and SOC medium preparation.
E.coli Competent Cells CJ236 Manual
TaKaRa JM109 Competent Cells Manual
User Manual 1 pages · 260 KB · ENThe TaKaRa JM109 Competent Cells manual provides preparation, transformation, and storage instructions for the E.coli JM109 strain.
E.coli Competent Cells DH5α Manual
Takara HST08 Premium Competent Cells Manual
User Manual 1 pages · 246 KB · ZHThe Takara HST08 Premium Competent Cells manual provides storage, preparation, and transformation instructions for high‑efficiency DNA uptake.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in E.coli manuals
How should the Electro‑Cell be stored before use?
Store the Electro‑Cell at –80 °C; after thawing it must not be refrozen.
What transport condition is required for the Electro‑Cell?
The Electro‑Cell must be transported using dry ice.
How many transformants can be obtained using 10 pg of pUC19 plasmid?
Approximately 1 × 10^8 transformants are obtained from 50 µL of cells with 10 pg DNA.
What are the main steps of the electroporation protocol?
Thaw the Electro‑Cell, add 1‑2 µL DNA, mix and load into a pre‑cooled 0.1 cm cuvette, apply 15 kV, recover in SOC medium, incubate, and plate on selective agar.
How should the Electro‑Cell be stored before use?
Store the Electro‑Cell at –80 °C and do not refreeze after thawing.
What is the maximum amount of DNA that can be added to a 50 µL Electro‑Cell?
Do not exceed 10 ng of DNA per 50 µL cell suspension.