E.coli Competent Cells BMH71-18 mutS is a highly efficient competent cell that can be used for gene recombination experiments, making gene libraries, recombinant plasmids and performing subcloning experiments.
The BMH71-18 competent cells are a Takara‑produced E.coli strain designed for high‑efficiency transformation of low‑quantity DNA, such as plasmid or phage DNA. The cells are supplied lyophilized and should be stored at –80 °C until use.
The manual details the thaw‑and‑transform procedure, including the recommended DNA amount (≤10 ng), incubation steps at 42 °C and 37 °C, and the composition of SOC recovery medium. Precautions such as using sterile water and avoiding repeated freeze‑thaw cycles are also provided. This manual is written in Japanese.
| Packaging amount | 7 g |
|---|---|
| Control DNA supplied | pUC119, 0.1 ng/µl |
| Cell concentration | 1‑2 × 10³ cells/µl |
| Transformation efficiency | > 1 × 10⁷ transformants/µg pUC119 |
| SOC medium composition | 2 % Bacto tryptone, 0.5 % Bacto yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgSO₄, 10 mM MgCl₂, 20 mM Glucose |
Store the cells at –80 °C and avoid refreezing after thawing.
SOC contains 2 % Bacto tryptone, 0.5 % Bacto yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgSO₄, 10 mM MgCl₂, and 20 mM glucose.