E.coli Competent Cells HST08 Premium is a high-efficiency host E.coli HST08 Premium that has a high transformation efficiency and can be used for the preparation of methylated plasmids. It is also suitable for the cloning of long fragments of 10 kbp and above. When it is used with Takara DNA Ligation Kit LONG (Takara Code: D6024), it works well. pUC series plasmids, BAC libraries, and other conversions can be used. β-galactosidase α-complementation can be used to add X-gal for blue-white screening to select positive clones.
The HST08 Premium competent cells are a high‑efficiency strain supplied by Takara for cloning applications, especially when target DNA is present at low concentrations. The product includes 100 µl aliquots (10 tubes) and a control pUC19 DNA (0.1 ng) for reference.
The manual details thawing, mixing with up to 10 ng of DNA, a 30‑minute ice incubation, a 45‑60 second heat‑shock at 42 °C, and recovery in SOC medium. It also lists storage at –80 °C, recommended tube types, and precautions to maintain transformation efficiency. This manual is written in Chinese.
| Packaging | 100 µl × 10 tubes |
|---|---|
| Control DNA | pUC19, 0.1 ng per tube (10 × 1) |
| Cell Concentration | 1–2 × 10³ cells/µl |
| Typical Transformation Efficiency | > 1 × 10⁸ transformants per µg pUC19 |
| Heat‑Shock Condition | 42 °C for 45–60 seconds |
| SOC Medium Composition | 2% Bacto tryptone, 0.5% Bacto yeast extract, 10 mM NaCl, 2.5 mM K₂HPO₄, 10 mM MgSO₄, 10 mM MgCl₂, 20 mM Glucose |
After adding DNA, incubate the mixture at 42 °C for 45–60 seconds.
SOC contains 2% Bacto tryptone, 0.5% Bacto yeast extract, 10 mM NaCl, 2.5 mM K₂HPO₄, 10 mM MgSO₄, 10 mM MgCl₂, and 20 mM glucose.
More than 1 × 10⁸ transformants per µg of pUC19 DNA are typically obtained.