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Takara JM109 Electro-Cells User Manual
The Takara JM109 Electro‑Cells are high‑efficiency competent E. coli cells supplied with 0.01 ng/µL pUC19 DNA as a control. They should be stored at –80 °C and thawed just before use. The manual details the genotype of the JM109 strain and provides guidelines for handling the cells.
The document describes a step‑by‑step electroporation procedure, including cell preparation, DNA addition, electroporation at 15 kV, recovery in SOC medium, and plating on selective agar. Additional protocols for M13 phage DNA preparation and a list of precautions (e.g., using sterile water, avoiding repeated freeze‑thaw cycles) are also included. This manual is written in Japanese.
TAKARA JM109 specifications
| Cell concentration | 1‑2 × 10^10 cells/mL |
|---|---|
| Control DNA in package | 0.01 ng/µL pUC19 |
| Typical transformation yield (10 pg DNA) | ~1 × 10^8 transformants per 50 µL |
| Electroporation voltage | 15 kV |
All TAKARA JM109 documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
How many transformants can be obtained using 10 pg of pUC19 plasmid?
Approximately 1 × 10^8 transformants are obtained from 50 µL of cells with 10 pg DNA.
What are the main steps of the electroporation protocol?
Thaw the Electro‑Cell, add 1‑2 µL DNA, mix and load into a pre‑cooled 0.1 cm cuvette, apply 15 kV, recover in SOC medium, incubate, and plate on selective agar.
What is the typical transformation efficiency using pUC19 DNA?
Approximately 1 × 10⁸ transformants are obtained per 1 pg of pUC19 plasmid DNA.
Is this product intended for clinical or therapeutic use?
No, the product is for research use only and not intended for therapeutic or diagnostic procedures.