Agilent Lambda ZAP II Undigested Vector Kit Instruction Manual

Lambda ZAP II Undigested Vector Kit instruction manual


Agilent Lambda ZAP II Undigested Vector Kit Instruction Manual - cover page
Brand
Agilent
Category
Other
Document type
User Manual
Language
English
Pages
33
File format
PDF
File size
266 KB
Published
19 October, 2023
Updated
19 October, 2023
MD5 checksum
A4D9E246172A533684C63173ED328B86

About this manual

This document is the instruction manual for the Lambda ZAP II Undigested Vector Kit (Catalog #236201), manufactured by Agilent Technologies. It provides comprehensive guidance on the materials provided, including the undigested Lambda ZAP II DNA, host strains (XL1-Blue MRF' and SOLR), and helper phages. The manual outlines specific storage conditions for the vector and bacterial stocks to ensure stability before use.

What's inside

  • Materials Provided
  • Storage Conditions
  • Overview of the Lambda ZAP II Vector System
  • Bacterial Host Strains
  • Establishing an Agar Plate Bacterial Stock
  • Preparing a -80°C Bacterial Glycerol Stock
  • Growth of Cells for Plating Phage
  • Determining Background by Color Selection
  • In Vivo Excision of the pBluescript Phagemid
  • Troubleshooting

Specifications

Vector Size40.82 kb
Insert Size Range0 to 10 kb
Unique Cloning Sites6
Vector Storage Temperature-20°C
Helper Phage Storage Temperature-80°C
Bacterial Glycerol Stock Storage-80°C

Frequently asked questions

What are the unique cloning sites in the Lambda ZAP II vector?

The vector has six unique cloning sites: Sac I, Not I, Xba I, Spe I, EcoR I, and Xho I.

Which bacterial host strains are included in the kit?

The kit includes XL1-Blue MRF' strain and SOLR strain as bacterial glycerol stocks.

What is the recommended storage temperature for the Lambda ZAP II Vector?

The Lambda ZAP II Vector should be stored at -20°C.

Can the Lambda ZAP II vector be used for diagnostic procedures?

No, the product is for research use only and not for use in diagnostic procedures.

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