This is the instruction manual for pBluescript II RI Predigested Vector, which introduces the product's usage methods, precautions and warranty policy.
This document provides instructions for the Agilent pBluescript II RI Predigested Vector, a high-copy-number pUC-based plasmid designed for convenient DNA insertion and blue-white screening. The vector is predigested with EcoR I and CIAP-treated to reduce background nonrecombinants, featuring a multiple cloning site with unique restriction sites and T3/T7 promoters for in vitro RNA synthesis.
| Vector Concentration | 20 ng/μl |
|---|---|
| Vector Quantity | 55 μl (1100 ng) |
| Test Insert Concentration | 10 ng/μl |
| Test Insert Quantity | 3 μl (30 ng) |
| Storage Temperature | -20°C |
| Vector Size | 3.0 kb |
| Recommended Transformation Efficiency | ≥5 × 109 cfu/μg DNA |
The vector is predigested with the EcoR I restriction enzyme.
Colonies containing vector without insert will be blue in the presence of X-gal and IPTG.
The test insert is a kanamycin-resistance gene used to verify the ability of the vector to ligate an insert.