This is a manual for Guinea pig SLC6A3 / Dopamine Transporter ELISA Kit.
This document provides detailed instructions for using the Guinea pig SLC6A3 / Dopamine Transporter ELISA Kit based on sandwich ELISA principles. It includes information on kit components, storage, required supplies, assay procedure, calculation of results, and troubleshooting. The kit is for research use only and not approved for diagnostic use. The manual also covers experimental layout, sample preparation, and validation steps to ensure accurate results.
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The assay is based on the sandwich ELISA principle, using a capture antibody, detection antibody, and HRP-conjugated avidin for color development at 450nm.
The Assay Plate, Standard, Detection Reagent A, and Detection Reagent B should be stored at -20°C. All other components should be stored at 4°C. TMB Substrate should never be frozen.
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.