This is a user manual for LS-F19374 Mouse GABBR1 / GABA B Receptor 1 ELISA Kit.
This document is the user manual for the LSBio LS-F19374 Mouse GABBR1 / GABA B Receptor 1 ELISA Kit. It is intended for research use only and is not approved for diagnostic use in humans. The kit utilizes a sandwich ELISA assay principle to detect the target antigen.
| Target | Mouse GABBR1 / GABA B Receptor 1 |
|---|---|
| Assay Principle | Sandwich ELISA |
| Detection Method | Colorimetric - 450nm |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials |
| Sample Diluent Volume | 1 vial x 20 ml |
| Assay Diluent A Volume | 1 vial x 12 ml |
| Assay Diluent B Volume | 1 vial x 12 ml |
| Detection Reagent A Volume | 1 vial x 120 µl |
| Detection Reagent B Volume | 1 vial x 120 µl |
| Wash Buffer Quantity | 1 vial x 20 ml (30 x) |
| TMB Substrate Volume | 1 vial x 9 ml |
| Stop Solution Volume | 1 vial x 6 ml |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The optical density (OD) of the well is measured at a wavelength of 450 nm ± 2 nm.
Upon receipt, the kit should be stored at 4 °C if intended for use within 24 hours.
Required equipment includes a microplate reader with a 450nm wavelength filter, high-precision pipette, sterile pipette tips, Eppendorf tubes, a 37 °C incubator, and deionized or distilled water.