This is a manual of a kit for Chicken Basigin / Emmprin / CD147 ELISA.
This document provides step-by-step instructions for using the Chicken Basigin / Emmprin / CD147 ELISA Kit based on the sandwich ELISA principle. It includes details on kit components, storage conditions, required equipment, assay procedure, calculation of results, and troubleshooting. The kit is for research use only and not approved for diagnostic or clinical use. It is intended for use with chicken samples and requires careful handling and validation before use.
The manual covers experimental layout, sample preparation, reagent preparation, assay steps, and interpretation of results. It also includes a troubleshooting guide for common issues such as poor standard curves, low signal, high background, and low sensitivity. Safety precautions and disposal instructions are provided, including warnings about sodium azide and sulfuric acid. Technical support is available via email.
| Target | Chicken Basigin / Emmprin / CD147 |
|---|---|
| Detection Method | Colorimetric - 450nm |
| Kit Storage | Assay Plate, Standard, Detection Reagent A, and Detection Reagent B stored at -20°C; other components at 4°C; TMB Substrate never frozen. |
| Sample Types | Chicken samples |
No, this kit is for Research Use Only and is not approved for diagnostic or clinical use.
The assay is colorimetric, measured at 450nm wavelength.
Store the Assay Plate, Standard, Detection Reagent A, and Detection Reagent B at -20°C; store other components at 4°C; never freeze TMB Substrate.
Microplate reader with 450nm wavelength filter, high-precision pipette, sterile pipette tips, Eppendorf tubes, 37°C incubator, deionized or distilled water, absorbent paper.