LSBioTM Human CD40L ELISA Kit User Manual
The LSBio Human sCD40 Ligand ELISA Kit is designed for the quantitative determination of natural or recombinant Human sCD40 Ligand concentrations in experimental samples such as cell lysates, serum, and plasma. The assay utilizes a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) format where the target protein is bound by capture antibodies coated to the well-bottom and detected by secondary antibodies conjugated with peroxidase.
| Target Analyte | Human sCD40 Ligand |
|---|---|
| Standard Concentration | 83 ng |
| Detection Wavelength | 450 nm |
| Correction Wavelength | 540 nm or 570 nm |
| Storage Temperature (Unopened) | 4°C |
| Storage Duration (Unopened) | 6 Months |
No, the kit is intended for research purposes only and is not approved for use in humans or for clinical diagnosis.
The kit can be used to determine concentrations in cell lysates, serum, and plasma.
A microplate reader capable of measuring absorbance at 450 nm with a correction wavelength of 540 nm or 570 nm is required.