This is a manual for Guinea pig PDXK / PNK ELISAKit. It provides detailed instructions on how to use the kit.
This document is the user manual for the LSBio LS-F47566 ELISA Kit, designed for the detection of Guinea pig PDXK/PNK. The kit operates on the Sandwich ELISA principle and is intended for research use only, not for diagnostic purposes in humans or clinical settings. It includes detailed instructions on assay planning, sample preparation, and the specific steps required to perform the colorimetric assay.
| Target | Guinea pig PDXK / PNK |
|---|---|
| Detection Range | Not specified in text |
| Detection Method | Colorimetric - 450nm |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials (Lyophilized) |
| Sample Diluent Volume | 1 vial x 20 ml |
| Assay Diluent A Volume | 1 vial x 12 ml |
| Assay Diluent B Volume | 1 vial x 12 ml |
| Detection Reagent A Volume | 1 vial x 120 µl |
| Detection Reagent B Volume | 1 vial x 120 µl |
| Wash Buffer Volume | 1 vial x 20 ml (30x) |
| TMB Substrate Volume | 1 vial x 9 ml |
| Stop Solution Volume | 1 vial x 6 ml |
| Plate Sealers Quantity | 4 |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The Assay Plate should be stored at -20°C if not intended for use within 24 hours.
A microplate reader with a 450nm wavelength filter, high-precision pipette, sterile tips, Eppendorf tubes, a 37°C incubator, and deionized or distilled water are required.
The TMB Substrate should never be frozen.