LSBio Bovine Vitamin D Receptor / VDR ELISAKit (Sandwich ELISA) User Manual

This is a manual of Bovine Vitamin D Receptor / VDR ELISAKit (Sandwich ELISA)


LSBio Bovine Vitamin D Receptor / VDR ELISAKit (Sandwich ELISA) User Manual - cover page
Brand
LSBio
Category
Health Care
Document type
User Manual
Language
English
Pages
25
File format
PDF
File size
495 KB
Published
18 October, 2023
Updated
18 October, 2023
MD5 checksum
A4C77B1162EE3E19E216C47F3D9B0033

About this manual

This manual provides detailed instructions for using the Bovine Vitamin D Receptor ELISA kit (Catalog No. LS-F14617) based on the sandwich ELISA principle. It includes sections on kit components, storage conditions, required equipment, sample preparation, assay procedure, and troubleshooting. The kit is for research use only and not approved for diagnostic use in humans.

The assay involves coating wells with capture antibody, adding standards or samples, then detecting bound antigen with biotin-conjugated antibody, avidin-HRP, and TMB substrate. Optical density is measured at 450nm to determine antigen concentration using a standard curve.

What's inside

  • Assay Specifications
  • Assay Principle
  • Kit Components
  • Kit Storage
  • Other Required Supplies
  • Assay Planning
  • Experimental Layout
  • Sample Collection
  • Sample Preparation
  • Reagent Preparation

Specifications

TargetBovine Vitamin D Receptor / VDR
Detection MethodColorimetric - 450nm
Kit Catalog NumberLS-F14617

Frequently asked questions

What is the detection method for this ELISA kit?

The detection method is colorimetric, measured at 450nm.

What is the target antigen for this kit?

The target antigen is Bovine Vitamin D Receptor / VDR.

Is this kit approved for diagnostic use?

No, this kit is for Research Use Only and not approved for diagnostic use in humans.

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