Chicken COL11A1 / Collagen XI Alpha 1 ELISA Kit (Sandwich ELISA) User Manual
This document is the user manual for the LSF42545 Chicken COL11A1 / Collagen XI Alpha 1 ELISA Kit manufactured by LSBio. The kit utilizes the Sandwich ELISA principle for the detection of Chicken COL11A1 in research settings. It is explicitly stated that the kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The manual provides comprehensive instructions on kit components, storage conditions, and required supplies such as a microplate reader and incubator. It details the experimental layout, sample preparation, and the step-by-step assay procedure. Additionally, it includes a troubleshooting guide for common issues like poor standard curves or high background noise.
| Target | Chicken COL11A1 / Collagen XI Alpha 1 |
|---|---|
| Detection Range | Not specified in text |
| Sensitivity | Not specified in text |
| Detection Method | Colorimetric - 450nm |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials |
| Wash Buffer Concentration | 30x |
| TMB Substrate Volume | 9 ml |
| Stop Solution Volume | 6 ml |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The Assay Plate should be stored at -20°C if not intended for use within 24 hours.
Required equipment includes a microplate reader with a 450nm wavelength filter, a 37°C incubator, and high-precision pipettes.
The assay is based on the sandwich ELISA principle using a capture antibody, detection antibody, and Avidin-HRP conjugate.