This manual is a reagent kit manual for detecting the Glutathione Reductase (GR) level in human.
This manual provides detailed instructions for using the Human GLRX / Glutaredoxin ELISA Kit (Catalog No. LS-F16805) for research purposes only. It includes assay principles, component lists, storage instructions, sample preparation, experimental layout, and troubleshooting guidance. The kit is based on the sandwich ELISA principle and uses colorimetric detection at 450nm. All reagents must be stored and handled according to specified conditions to ensure accurate results.
The manual also outlines sample collection and preparation methods for various biological samples including breast milk, cell lysates, plasma, and serum. It includes troubleshooting tips for common issues such as poor standard curves, low signal, and high background, with solutions based on pipetting accuracy, incubation time, and wash procedures.
| Target | Glutathione Reductase (GR) |
|---|---|
| Detection Method | Colorimetric - 450nm |
| Sample Types | Human samples including breast milk, cell lysates, plasma, serum, etc. |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The assay uses a colorimetric detection method at 450nm.
The kit is intended for use with human samples such as breast milk, cell lysates, plasma, serum, and others as specified in the manual.