This is a manual for the Rat HADH ELISAKit. It includes the assay principle, kit components, storage, assay planning, experimental layout, sample collection, sample preparation, standard preparation, reagent preparation, assay procedure, calculation of results, troubleshooting guide, assay usage and support, returns, refunds, cancelations.
This document is the user manual for the LSBio Rat HADH ELISA Kit (Catalog No. LS-F17792). The kit utilizes the Sandwich ELISA principle to detect Rat HADH in various sample types. It is intended for research use only and is not approved for diagnostic use in humans.
The manual details the assay specifications, principle, kit components, and storage requirements. It provides step-by-step instructions for sample collection and preparation, standard preparation, reagent preparation, and the complete assay procedure. Additionally, it includes guidelines for experimental layout, calculation of results, and a troubleshooting guide.
| Target | Rat HADH |
|---|---|
| Assay Principle | Sandwich ELISA |
| Detection Method | Colorimetric - 450nm |
| Wavelength | 450 nm ± 2 nm |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials |
| Sample Diluent Quantity | 1 vial x 20 ml |
| Assay Diluent A Quantity | 1 vial x 12 ml |
| Assay Diluent B Quantity | 1 vial x 12 ml |
| Detection Reagent A Quantity | 1 vial x 120 µl |
| Detection Reagent B Quantity | 1 vial x 120 µl |
| Wash Buffer Quantity | 1 vial x 20 ml |
| TMB Substrate Quantity | 1 vial x 9 ml |
| Stop Solution Quantity | 1 vial x 6 ml |
| Plate Sealers Quantity | 4 |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The optical density (OD) of the well is measured at a wavelength of 450 nm ± 2 nm.
You need a microplate reader with a 450nm wavelength filter, high-precision pipette, sterile pipette tips, Eppendorf tubes, a 37 ° C incubator, and deionized or distilled water.