This is an ELISA kit for detecting rabbit SOX1 protein.
This document provides step-by-step instructions for using the Rabbit SOX1 ELISA Kit based on sandwich ELISA principles. It details kit components, storage conditions, required supplies, experimental layout, sample preparation, assay procedure, and troubleshooting. The kit is for research use only and not approved for clinical diagnosis. The manual includes guidance on standard curve generation, sample dilution, and handling potential issues during the assay.
The kit contains pre-coated microtiter plates, standards, diluents, detection reagents, and substrates. All reagents must be stored under specified conditions to maintain integrity. The assay uses colorimetric detection at 450nm and requires a microplate reader, pipettes, and incubator. Validation steps are recommended before using valuable samples.
| Target | Synonyms: [Synonyms] Specificity: [Target Species] [Target Common Name] |
|---|---|
| Detection Method | Colorimetric - 450nm |
| Kit Components | Coated 96-well Strip Plate, Standard (Lyophilized), Sample Diluent, Assay Diluent A, Assay Diluent B, Detection Reagent A, Detection Reagent B, Wash Buffer (30x), TMB Substrate, Stop Solution, Adhesive Plate Sealers, Instruction Manual |
The kit uses a colorimetric detection method at 450nm.
This kit is for Research Use Only and is not intended for diagnostic use. It is not approved for use in humans or for clinical diagnosis.
A microplate reader with 450nm wavelength filter, high-precision pipette, sterile pipette tips, Eppendorf tubes, 37°C incubator, deionized or distilled water, and absorbent paper.