This is a manual for Bovine TLR3 ELISAKit (Sandwich ELISA). It includes the kit components, assay principle, assay procedure, calculation of results, troubleshooting guide, etc.
This document is the user manual for the LSF18986 Bovine TLR3 ELISA Kit, which utilizes the Sandwich ELISA principle for the detection of TLR3 in bovine samples. The kit is intended for research use only and is not approved for diagnostic use in humans or clinical diagnosis.
The manual covers the entire workflow, including assay specifications, kit components, storage conditions, and required supplies. It provides detailed instructions for sample collection from various sources such as plasma, serum, and cell lysates, as well as standard preparation and the step-by-step assay procedure. It also includes a troubleshooting guide for common issues like poor standard curves or high background.
| Detection Method | Colorimetric - 450nm |
|---|---|
| Wavelength | 450 nm ± 2 nm |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials |
| Sample Diluent Volume | 1 vial x 20 ml |
| Assay Diluent A Volume | 1 vial x 12 ml |
| Assay Diluent B Volume | 1 vial x 12 ml |
| Detection Reagent A Volume | 1 vial x 120 µl |
| Detection Reagent B Volume | 1 vial x 120 µl |
| Wash Buffer Quantity | 1 vial x 20 ml (30 x) |
| TMB Substrate Volume | 1 vial x 9 ml |
| Stop Solution Volume | 1 vial x 6 ml |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The assay is based on the sandwich ELISA principle where a target antigen binds to a capture antibody, followed by a biotin-conjugated detection antibody and an Avidin-HRP conjugate.
Required equipment includes a microplate reader with a 450nm filter, high-precision pipettes, sterile pipette tips, Eppendorf tubes, a 37 °C incubator, and deionized or distilled water.