This manual introduces the use of LSBio's SCG2 / Secretogranin II ELISA Kit, including the kit composition, kit storage, sample collection, sample preparation, standard preparation, reagent preparation, experimental steps, and result calculation.
This user manual provides detailed instructions for using the Pig SCG2/Secretogranin II ELISA Kit (Catalog No. LS-F19743) based on the sandwich ELISA principle. It includes sections on kit components, storage conditions, required supplies, assay planning, experimental layout, sample collection and preparation, reagent preparation, assay procedure, result calculation, and troubleshooting. The kit is for research use only and not approved for diagnostic use.
The manual covers sample types such as plasma, serum, cell lysates, and breast milk, with specific protocols for each. It also details reagent preparation, incubation steps, and how to interpret results using an OD standard curve. Troubleshooting guidance addresses common issues like poor standard curves, low signal, high background, and contamination.
| Target | Pig SCG2/Secretogranin II |
|---|---|
| Detection Method | Colorimetric - 450nm |
| Assay Principle | Sandwich ELISA |
| Catalog Number | LS-F19743 |
The kit uses a colorimetric detection method at 450nm.
It is based on the sandwich ELISA principle, where a capture antibody binds the target antigen, followed by detection antibody and enzyme conjugate.
The kit is intended for use with samples such as [Target Species] [add samples types].