This is a manual for a kit for TRXR1 / TXNRD1 ELISA assay
This document provides detailed instructions for using the Rat TRXR1 / TXNRD1 Sandwich ELISA kit from LSBio. It covers assay principles, kit components, storage conditions, required equipment, sample preparation, experimental layout, assay procedure, result calculation, and troubleshooting. The kit is for research use only and not approved for diagnostic use.
The assay uses a sandwich ELISA method with pre-coated capture antibodies, biotin-conjugated detection antibodies, and an HRP-conjugated avidin for colorimetric detection at 450nm. Kit components include a coated plate, standards, diluents, detection reagents, wash buffer, substrate, and stop solution. Storage requirements vary by component, with some needing refrigeration and others freezing. The manual also includes troubleshooting guidance for common issues like poor standard curves or low signal.
| Target | [Target Common Name] |
|---|---|
| Sample Types | [Target Species] [add samples types] |
| Detection Method | Colorimetric - 450nm |
The detection method is colorimetric, measured at 450nm.
This kit is for research use only and not approved for diagnostic use.