This is a document that describes the assay principle for the Monkey FABP7 / BLBP / MRG ELISAKit. The kit is for research use only and not for diagnostic use.
This document is the user manual for the LSBio Monkey FABP7/BLBP/MRG ELISA Kit, catalog number LS-F43746. It describes a sandwich ELISA assay designed for the detection of FABP7, BLBP, and MRG antigens in monkey samples. The kit is intended for research use only and is not approved for diagnostic use in humans or clinical settings.
The manual provides comprehensive instructions on assay planning, experimental layout, sample preparation, and the step-by-step assay procedure. It includes a list of kit components, storage conditions, required supplies, and a troubleshooting guide to address common issues such as poor standard curves or high background noise.
| Target | FABP7, BLBP, MRG |
|---|---|
| Assay Principle | Sandwich ELISA |
| Detection Wavelength | 450 nm |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials |
| Sample Diluent Volume | 20 ml |
| Assay Diluent A Volume | 12 ml |
| Assay Diluent B Volume | 12 ml |
| Detection Reagent A Volume | 120 µl |
| Detection Reagent B Volume | 120 µl |
| Wash Buffer Concentration | 30x |
| TMB Substrate Volume | 9 ml |
| Stop Solution Volume | 6 ml |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The Assay Plate should be stored at -20°C if not intended for use within 24 hours.
The optical density (OD) of the well should be measured at a wavelength of 450 nm ± 2 nm.
Check pipettes for inaccuracies and ensure the standard powder is dissolved thoroughly by gentle mixing.