This is a pdf file. It is a product manual. It mainly introduces the features of the product, including brand, model and other information.
This document is a datasheet for the Agilent Bio Series Poly F, a proprietary polymeric fluorocarbon HPLC packing material designed for the reversed-phase separation of biological macromolecules. It features a 20-micron particle size with 300A pores and is chemically stable across a wide pH range, making it suitable for separating proteins and peptides in both acidic and basic mobile phases where standard silica columns may fail.
| Particle Size | 20-micron |
|---|---|
| Pore Size | 300A |
| Pressure Limit | 135 bars (2000 psi) |
| Flow Rate Limit | 6 mL/min |
| Maximum Temperature | 100° C |
| Guard Column Quantity | 2 grams |
Yes, it can be used with mobile phases between pH 11 and 13, unlike bonded silica packings.
This could be caused by a partially plugged column inlet frit or a void on the top of the column bed.