This document is the Agilent Ultron ES-OVM Method Development Protocol, which introduces the method development process for acidic, basic, and non-protolytic compounds, and gives typical examples.
This document provides a flowchart-based protocol for developing chromatographic methods on the Ultron ES-OVM stationary phase. It guides users through selecting appropriate mobile phases, such as phosphate buffers with modifiers like ethanol or acetonitrile, based on whether the target compounds are acidic or basic/nonprotolytic.
The document lists acetonitrile, ethanol, methanol, and 2-propanol as acceptable uncharged modifiers.
The protocol specifies a maximum limit of 50% for any added uncharged modifier.