Bio-Monolith QA columns are high-performance monolithic columns that offer all the advantages of a specially designed, continuous short polymeric bed. Their inherent features enable highly reproducible separations of macro bio-molecules, large proteins, DNA, and viral particles, at extremely high speeds. These columns are prepacked in dedicated stainless steel housings and allow user-friendly ( and quick ) connections to HPLC equipment. The information in this data sheet is being provided to ensure proper product care and maximal product lifetime.
This document is a datasheet for the Agilent Bio-Monolith QA Column, catalog number 5069-3636. It describes high-performance monolithic columns designed for the rapid separation of macro-biomolecules, large proteins, DNA, and viral particles using HPLC equipment.
The text provides basic characteristics such as column chemistry (strong anion exchanger), matrix dimensions, dynamic binding capacity, and maximum pressure limits. Additionally, it offers detailed instructions on taking care of the column, including regeneration procedures, Cleaning in Place (CIP) protocols for both regular and rigorous cleaning, and guidelines for long-term storage.
| Catalog number | 5069-3636 |
|---|---|
| Column chemistry | Strong anion exchanger; quaternary amine |
| Matrix dimensions (Diameter) | 5.2 mm |
| Matrix dimensions (Length) | 4.95 mm |
| Bed volume (CV) | 0.10 mL |
| Dynamic binding capacity | = 25 mg BSA/mL wet support |
| Maximum loading capacity | 1.2 to 14 mg |
| Recommended working flow rates | 0.2 to 2 mL/min |
| Maximum allowed pressure | 150 bar (15 MPa, 2200 psi) |
| Working temperature range | 4 to 40 °C |
The recommended pH range is 2 to 13.
Wash with at least 1 mL of DI water, then flush with at least 2 mL of a 20% ethanol solution.