This white paper introduces Agilent Oligonucleotide Array-Based Comparative Genomic Hybridization (CGH) technology, a highly sensitive technology for analyzing genomic and chromosomal abnormalities. The technology is based on the Agilent DNA Microarray platform, which can analyze copy number changes of genomic DNA to perform chromosome analysis.
The document introduces the Agilent DNA Microarray Platform, which utilizes oligonucleotide microarrays to revolutionize comparative genomic hybridization (CGH). It addresses limitations of traditional methods and BAC/cDNA arrays by offering higher resolution, flexibility, and sensitivity for detecting chromosomal variations.
The arrays are compatible with tissues, cell lines, whole blood, and other challenging samples.
The overall median probe spacing is 8.9 kb.
The experiment used 1.5 yg of labeled DNA.