Stratagene Mutagenesis Solutions for Your Protein Engineering Needs
Stratagene offers a comprehensive portfolio of reagents and kits designed to help researchers maximize experiment time while maintaining high efficiency and accuracy in targeted protein engineering. As an Agilent Technologies company, Stratagene provides innovative tools such as the QuikChange® site-directed mutagenesis and GeneMorph® random mutagenesis kits.
The document details specific applications for various kits, including the QuikChange Lightning kit for rapid protocols under three hours, the QuikChange II XL kit for larger plasmids over 8 kb, and the GeneMorph II kit for creating large mutant libraries. It also explains the underlying methods, such as the linear amplification strategy of QuikChange to minimize errors.
The kit is suitable for DNA templates up to 14 kb.
The protocol can be completed in less than three hours plus an overnight transformation.