The document describes the features and characteristics of a series of bioreagent components for processing microarrays produced by Agilent Bioreagent, including Total RNA Isolation Mini Kit, Plant RNA Isolation Mini Kit, and Low Input Quick Amp Labeling Kit.
This document serves as an ordering guide for Agilent Bioreagent Components designed for processing microarrays. It lists various reagents under categories such as Gene Expression and miRNA Reagents, and CGH, ChIP-on-Chip, and DNA Methylation Reagents. The guide provides part numbers and brief descriptions for kits used in total RNA isolation, labeling, hybridization, and wash procedures.
| Total RNA Isolation Mini Kit capacity | 50 total RNA isolations |
|---|---|
| Low Input Quick Amp Labeling Kit, one-color input | 25 — 200 ng total RNA input |
| Low Input Quick Amp Labeling Kit, two-color reactions | 48 reactions |
| Quick Amp Labeling Kit, one-color reactions | 20 reactions |
| One-Color RNA Spike-in Kit capacity | at least 200 microarray hybridizations |
| Gene Expression Hybridization Kit capacity | at least 10 slides |
| Gene Expression Wash Buffer Kit capacity | up to 80 slides |
| miRNA Complete Labeling and Hybridization Solution capacity | 24 microarrays |
| Universal Human Reference RNA contents | 2 tubes x 200 yg each |
| Agilent RNA 6000 Nano Kit sample capacity | 300 samples |
| Agilent DNA 1000 Kit fragment range | 25 to 1000bp |
| Agilent DNA 12000 Kit fragment range | 100 to 12000bp |
| Genomic DNA Enzymatic Labeling Kit capacity | 50 reactions |
| Genomic DNA High-Throughput ULS Labeling Kit capacity | 96 — 8-pack microarrays |
| Oligo aCGH/ChIP-on-Chip Hybridization Kit (25) capacity | 25 slides |
| Oligo aCGH Wash Buffer Kit Option 1 capacity | up to 40 oligo aCGH slides |
| Oligo aCGH Wash Buffer Kit Option 2 capacity | up to 120 oligo aCGH slides |
It uses 25 — 200 ng total RNA input.
The kit is sufficient for 20 reactions.
It is sufficient for at least 200 microarray hybridizations.
It is for sizing and quantitation of dsDNA fragments from 25 to 1000bp.