Agilent's dual-mode Gene Expression Microarray platform is a comprehensive and robust system that couples the ease and simplicity of one-color experimental design with the capability of two-color to detect small transcriptional changes – all on the same platform.
Agilent's One-Color Gene Expression Microarray Solution combines the simplicity of one-color experimental design with the detection capabilities of two-color systems. This dual-mode platform is designed to expand microarray applications while reducing system variability and accelerating discovery processes.
The solution features high-performance inkjet synthesis producing 60-mer oligonucleotide probes that detect very low-abundance transcripts, even with as little as 200 ng of total RNA input. It offers exceptional linear dynamic range, array-to-array reproducibility, and the ability to resolve small transcriptional changes.
| Oligonucleotide probe length | 60-mer |
|---|---|
| Starting sample input Total RNA | 200 ng (single round) |
| Type of labeling | Cy3-labelled linear amplification |
| Linear dynamic range | 3.5-4.0 orders of magnitude |
| Total dynamic range | nearly 4.5 orders of magnitude |
The system requires as little as 200 ng of total RNA for a single round of amplification.
The probes used in this solution are 60-mers.