High Sensitivity Capillary HPLC with Diode-array Detection
This document describes how to perform high-sensitivity capillary HPLC analysis using the Agilent 1100 Series HPLC high pressure gradient system. It details the prerequisites for running capillary columns, including the need for low system delay volume and specific detector cells.
| Column internal diameter | 180 to 300 µm |
|---|---|
| Flow rates | 2 to 4 µL/min |
| Column length | 100 to 200 mm |
| Flow cell volume | 500 nL |
| Flow cell pathlength | 10 mm |
| Analysis range | femtomole range |
The system requires low system delay volume, the addition of a flow splitter, and the availability of nL detector cells.
It allows spectra of eluting compounds to be stored digitally and manipulated by algorithms, which is useful for identifying peptides with aromatic amino acids.