This paper introduces a novel capillary LC system with a unique pump design and electronic flow control. The system is capable of high performance peptide separations on a 300-µm id column with a flow rate of 5.5 µL/min.
The document addresses the reputation of capillary LC as being unreliable and demonstrates the performance of a new Agilent capillary LC system. It highlights a unique pump design with electronic flow control that achieves excellent repeatability for routine analysis, matching the performance of standard LC equipment.
| Flow Cell Volume | 500 nL |
|---|---|
| Column Part Number | 5064-8265 |
The retention time repeatability achieved is less than 0.5% RSD.
The peptide separations were performed using a flow rate of 5.5 uL/min.
The analysis achieved excellent repeatability data on retention time with less than 0.5 % RSD.
The detection system uses a flow cell with a 10-mm path length.