Zorbax Eclipse XDB-C8 is a high performance ion-pairing column for the analysis of polar compounds. The column uses double endcapping technology, which makes it stable in high pH mobile phases.
The document presents data on the analysis of nucleic acids, specifically nucleotides, in biochemistry and medicine. It highlights the need for rapid determination of base pair composition of DNA and RNA hydrolysates.
A specific application is detailed using a ZORBAX Eclipse XDB-C8 column (3.5 um, 4.6 x 75 mm). The method involves analyzing nucleotides at an elevated pH of 7.0 to prevent degradation, utilizing double-endcapped silica surface stability.
It is used to analyze nucleotides via ion-pairing chromatography at elevated pH.
The column has dimensions of 4.6 x 75 mm.
The recommended injection volume is 1 pL.