This article introduces the features of ZORBAX® Rapid Resolution reversed-phase columns, which can reduce analysis time and solvent usage by 40 to 66 percent (depending on column size), while maintaining equivalent resolution to 5-μm particle sized columns. Rapid Resolution columns with 3.5-μm particles and short column lengths (150, 75, and 50 mm) provide the same separating power as 250- and 150-mm columns using 5-μm particles. They have the same physical characteristics (surface area, pore size, and surface coverage) as 5-μm particles, resulting in the same selectivity and retention times. ZORBAX StableBond Rapid Resolution columns, with both 300 Å and 80 Å pore sizes, use a proprietary bonding technology for stable operation at low pH and high temperature, making them ideal for protein and peptide separations.
ZORBAX Rapid Resolution reversed-phase columns utilize 3.5-um particles in short column lengths (150, 75, and 50 mm) to reduce analysis time and solvent usage by 40 to 66 percent while maintaining equivalent resolution to standard 5-um particle columns. These columns are designed for applications such as high-throughput sample testing, complex tryptic digest resolution, and protein/peptide separation.
| Particle Size | 3.5-um |
|---|---|
| Column Lengths Available | 150, 75, and 50 mm |
| Analysis Time Reduction | 40 to 66 percent |
| Operating Temperature (C18) | up to 90 °C |
| Operating Temperature (C8, C3, CN) | up to 60 °C |
Analysis time is reduced by 40 to 66 percent depending on column size.
Yes, they provide equivalent resolution to 5-um particle sized columns.