The method is based on the use of a mixed 119Sn-enriched spike and uses GC-MS coupled with electron impact ionization to simultaneously determine monobutyl, dibutyl, and tributyltin in water, sediments, and biota.
The document presents an application note detailing the development of a speciation method for the simultaneous determination of monobutyltin (MBT), dibutyltin (DBT), and tributyltin (TBT) in environmental samples, including waters, sediments, and biota. The method utilizes gas chromatography-mass spectrometry (GC-MS) with electron impact ionization and is based on the use of a mixed spike containing 120Sn-enriched MBT, DBT, and TBT for isotope dilution analysis.
The procedure covers sample preparation steps such as extraction from sediments and mussel tissue using acetic acid/methanol mixtures, followed by ethylation with sodium tetraethylborate. Water samples are also processed via liquid-liquid extraction into hexane. The resulting derivatives are analyzed on an Agilent Technologies gas chromatograph model 6890N equipped with an HP-5MS capillary column and an Agilent mass spectrometric detector model 5973 Network MSD.
| Gas Chromatograph Model | Agilent Technologies gas chromatograph model 6890N |
|---|---|
| Mass Spectrometer Model | Agilent mass spectrometric detector model 5973 Network MSD |
| Column Type | HP-5MS capillary column (cross-linked 5% phenyl methyl siloxane, 30 m x 0.25 mm id x 0.25 um coating) |
| Carrier Gas | Helium with a constant flow of 1.2 mL/min |
| Ionization Energy | 70 eV |
The method allows for the simultaneous determination of monobutyl-, dibutyl-, and tributyltin in environmental samples.
The method uses electron impact ionization.