A liquid chromatography/mass spectrometry method using an electrospray ionization source in positive ion mode was developed for the analysis of glyphosate (N-phosphonomethyl glycine) and its metabolite, aminomethyl phosphonic acid in water. Both glyphosate and its metabolite were derivatized using 9-fluorenyl-methyl chloroformate in buffer solution prior to reversed phase high performance liquid chromatography separation. The method cleanly resolved both target molecules with excellent sensitivity in both positive and negative ion modes.
This document presents a liquid chromatography/mass spectrometry (LC/MS) method developed by Agilent Technologies for the analysis of glyphosate and its metabolite, aminomethyl phosphonic acid (AMPA), in environmental water samples. The method utilizes pre-column derivatization with 9-fluorenylmethyl chloroformate (FMOC) to enhance detection sensitivity.
The procedure details sample preparation steps involving buffer solutions and reagent mixing, followed by analysis using an Agilent 1100 LC/MS system equipped with electrospray ionization. The document provides specific instrument parameters, chromatography conditions, and presents results demonstrating the method's ability to resolve both target molecules in positive and negative ion modes.
The method requires a 100-mL water sample size to achieve adequate sensitivity.
The instrument can easily measure between 10 and 20 ng on column.