This document describes an improved chromatographic method for monitoring the bisphenol-A manufacturing process using Agilent RRHT Eclipse XDB-C18 columns. These columns use 1.8-µm particles versus conventional 3.5-µm or 5-µm particles, allowing seven times faster analyses, improved resolution, and higher sensitivity.
This application note details an improved chromatographic method for monitoring the Bisphenol-A (BPA) manufacturing process. The method utilizes Agilent RRHT Eclipse XDB-C18 columns, which feature 1.8-µm particles compared to conventional 3.5-µm or 5-µm particles. This technology allows for significantly faster analyses, improved resolution, and higher sensitivity.
| Mobile Phase A | 0.1% formic acid |
|---|---|
| Mobile Phase B | ACN: MeOH {200:800} |
| Flow Rate | 1 mL/min |
| Temperature | 25 °C |
| Gradient Type | linear, no isocratic holds |
| Original Analysis Time | 50 min |
| RRHT Analysis Time | 7.5 min |
The analysis time is reduced at least six-fold, from 50 minutes to 7.5 minutes.
Solvent consumption is reduced about 12.5 times, from 100 mL/analysis to only 7.5 mL/analysis.
The peak shape is more symmetrical because the Eclipse XDB-C18 column provides a more Gaussian peak shape, which is important for accurate quantification.