This document describes the use of vitamin E (tocopherol), phenolic antioxidants, and erucamide slip additives in polypropylene homopolymer formulations, as well as their analysis and detection using liquid chromatography with ultraviolet/visible detection. The Agilent 1200 Rapid Resolution LC system with Agilent ZORBAX RRHT columns allows for rapid separation of these antioxidants with the same or improved resolution. The Agilent method translator can be used to convert the ASTM method into new methods based on instrument parameters, column dimensions, and particle size in three modes: simple conversion, speed optimized, and resolution optimized methods.
The document details a method for resolving Vitamin E, phenolic antioxidants, and erucamide slip additives in polypropylene homopolymer formulations. It compares the performance of Agilent ZORBAX Eclipse XDB-C18 and XDB-C8 stationary phases, concluding that the C8 phase offers better retention characteristics and peak shape.
The text explains how to use the Agilent Method Translator to convert conventional ASTM methods into new methods optimized for speed or resolution on sub-two-micron columns. It covers three conversion modes: simple conversion, speed optimization, and resolution optimization.
| System Components | G1379B micro vacuum degasser, G1312B binary pump SL, G1367C high-performance autosampler SL, G1316B thermostatted column compartment SL, G1315C UV/VIS diode array detector SL |
|---|---|
| Software Version | ChemStation 32-bit version B.02.01-SR1 |
| Mobile Phase A | water |
| Mobile Phase B | acetonitrile (ACN) |
| Flow Rate (Standard) | 1.5 mL/min |
| Wavelength | 200 nm |
The ZORBAX Eclipse XDB-C8 column showed better retention capability and peak shape compared to the C18 column.
An injection volume of 5 uL was found suitable to avoid solvent influence and peak splitting observed at 10 uL.