A simple and effective method has been developed for the simultaneous determination of the seven tetracycline antibiotic residues in chicken meat.
This document details a validated method for the simultaneous determination of seven antibiotic residues (minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline, methacycline, and doxycycline) in chicken tissue. The method utilizes solid-phase extraction (SPE) with Agilent SampliQ OPT cartridges followed by high-performance liquid chromatography (HPLC) with UV detection.
The procedure covers sample preparation using EDTA-Mcllvaine buffer, SPE purification steps, and specific HPLC conditions including column type, flow rate, and gradient elution. Validation results demonstrate acceptable recoveries ranging from 59.0% to 99.0% and limits of detection between 2.5 and 5 µg/kg, meeting Chinese regulatory standards.
| Column dimensions | 250 mm x 4.6 mm id |
|---|---|
| Flow rate | 1.5 mL/min |
| Detector wavelength | 350 nm |
| Injection volume | 100 µL |
| Limits of detection | 2.5 to 5 µg/kg |
| Linear calibration range | 25 to 500 µg/kg |
| Recovery range | 59.0% to 99.0% |
The method determines minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline, methacycline, and doxycycline.
The method uses an Agilent SampliQ OPT cartridge.
The column temperature is set to 30 °C.
The mobile phase consists of methanol, acetonitrile, and 10 mmol/L TFA solution with gradient elution.