This Application Note presents a method for rapid determination of carbohydrates in food using capillary electrophoresis with indirect UV-detection. Non-derivatized carbohydrates can rapidly be determined using this method, which is simple and stable, and suitable for application in routine labs.
This document presents a method for the rapid monitoring of carbohydrates in processed foods, such as fruit juices and yogurt, using capillary electrophoresis (CE) with indirect UV-detection. The technique is highlighted for its simplicity, stability, and suitability for routine laboratory analysis, offering a viable alternative to high-performance liquid chromatography (HPLC) for complex sample matrices. The method allows for the determination of non-derivatized carbohydrates, which is important for regulatory compliance and consumer protection regarding additives like sweeteners and preservatives.
| Detection limits | 7-15 ppm |
|---|---|
| Capillary length (total) | 80.5 cm |
| Capillary effective length | 72 cm |
| Capillary internal diameter | 50 pm |
| Voltage | -25 kV |
| Temperature | 15°C |
| Detection signal | 350/20 nm |
The analysis uses the Agilent Capillary Electrophoresis system equipped with DAD detection and Agilent ChemStation software.
The standard assay was linear over the range 50-10 000 ppm with r² > 0.999.
Repeatability was < 0.16 % RSD for migration times and <2.3 % RSD for peak areas (n = 6).