This application note describes a method for authenticating Scotch whisky using GC analysis. The method involves analyzing the alcohol components in the whisky to determine its authenticity.
This document details the authentication of Scotch whisky using Gas Chromatography (GC) analysis with a Flame Ionization Detector (FID). The primary focus is on the Agilent J&W CP-Wax 57 CB Basic column, which is highlighted for its ability to separate critical alcohol congener peak pairs, such as 2-methyl-1-butanol and 3-methyl-1-butanol, and to resolve furfural from acetic acid.
The text provides specific operating conditions for the GC-FID technique, including column dimensions, carrier gas flow, injector temperature, and detector temperature. It compares the chromatographic profiles of genuine blended Scotch whisky against counterfeit products, noting differences in relative concentration levels and peak ratios that indicate the origin of the spirit.
| Column Dimensions | 50 m x 0.25 mm x 0.20 pm |
|---|---|
| Sample Volume | 0.5 uL |
| Carrier Gas | 3.0 mL/min H2 |
| Injector Temperature | 200 °C |
| Split Ratio | 1:25 |
| Column Temperature Program | 35 °C. 6 °C/min, 120 °C |
| Detector Type | FID |
The Agilent J&W CP-Wax 57 CB Basic column is used for the analysis.
It is highly suited for alcohol congener identification and separates critical peak pairs like 2-methyl-1-butanol and 3-methyl-1-butanol.
The column separates 2-methyl-1-butanol and 3-methyl-1-butanol, Isobutanol and isoamyl acetate, and Ethylacetate and acetal.