Agilent Volatile Organic Compounds in Serum or Plasma Application Note

A sensitive and reproducible gas chromatographic method for ethanol and other volatile organic compounds in serum or plasma was developed using the polar CP- Wax 52 CB column, creating good peak shapes; this can also be seen for ethylene glycol.


Agilent Volatile Organic Compounds in Serum or Plasma Application Note - cover page
Brand
Agilent
Category
Other
Document type
Application Note
Language
English
Pages
2
File format
PDF
File size
104 KB
Published
12 June, 2012
Updated
30 September, 2023
MD5 checksum
B92BA5ACADA41442EE84BE03C21B7F7C

About this manual

This document presents a sensitive and reproducible gas chromatographic method for analyzing alcohols, acetaldehyde, and acetone in serum and plasma. The method is particularly useful for forensic and toxicology laboratories and involves the direct injection of biological specimens with minimal pre-treatment. It specifically addresses the analysis of ethylene glycol, which can cause toxic effects such as renal dysfunction.

What's inside

  • Introduction
  • Sample Preparation
  • Calculation
  • Conditions
  • Peak Identification
  • Remarks

Specifications

ColumnCP-WAX 52 CB, 0.25 mm x 30 m: 0.5 um
Part NumberCP8746
Carrier GasNitrogen
Flow Rate1.46 mL/min
Detector Temperature250 °C
Detection Limit0.1 g/L

Frequently asked questions

What is the correction factor for ethanol concentration in plasma compared to whole blood?

The ethanol concentration of plasma is 1.17 times the concentration in whole blood.

How often should the carbon fritted liner be cleaned or changed?

It should typically be cleaned or changed after about 50 injections to prevent performance decrease.

Is this product approved for use in clinical diagnostic procedures?

No, this product is not cleared or approved by the FDA or other international regulatory agencies for use in clinical diagnostic procedures.

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