This article describes the method of analyzing volatile amines using the Agilent J&W Select CP-Volamine GC Column. The column is coated with a base deactivated non-polar siloxane type stationary phase, which provides a highly inert coating surface with minimal absorption for difficult basic compounds. The column exhibits good peak shape and excellent stability, making it suitable for the analysis of highly volatile and basic substances.
This document details the use of the Agilent J&W Select CP-Volamine WCOT fused silica column for the separation of volatile amines. The column features a base deactivated non-polar siloxane stationary phase optimized for these basic analytes, offering high inertness and stability for aqueous samples.
The note provides specific chromatographic conditions for Gas Chromatography with Flame Ionization Detection (GC-FID). It includes a table identifying 22 specific volatile amines and their retention times, along with a chromatogram illustrating the separation of a complex mixture.
| Column Type | WCOT fused silica |
|---|---|
| Stationary Phase | base deactivated non-polar siloxane |
| Part Number | CP7447 |
| Sample Solvent | Water |
| Carrier Gas | Helium |
| Carrier Gas Flow | 2.0 mL/min |
| Injector Temperature | 200 °C |
| Injection Volume | 0.5 wL |
| Detection Method | FID |
| Detector Temperature | 240 °C |
The column is optimized for the separation of volatile amines, such as methylamine and dimethylamine.
It is a base deactivated non-polar siloxane type stationary phase.