Agilent Cary 600 FTIR Series Quantitative Analysis Application Note

This document introduces the application of ATR-FTIR spectroscopy for quantitative analysis. The use of ATR accessories with Fourier transform infrared (FTIR) spectrometers is now commonplace. This accessory allows for the non-destructive measurement of samples with little or no preparation. Most samples can be directly applied to the internal reflection element (IRE) of an ATR without time-consuming dilution with matrices such as Nujol or KBr. The ATR accessory also allows for easy analysis of liquid samples with just a single drop required, applied directly to the IRE crystal. However, by the nature of their design, ATR accessories absorb infrared radiation and consequently reduce the amount of energy that reaches the infrared detector. The attenuation caused by these accessories typically varies from 70–90%. Agilent Cary 600 FTIR series spectrometers provide the ideal platform for this accessory by delivering up to four times more IR power at the sample focus than most competitive products. They have been designed to offset accessory attenuation of the IR beam and to provide exceptional performance and sensitivity, which are typically compromised with the use of the accessory. Infrared spectroscopy is particularly well-suited to quantitative analyses. The determination of sample concentration is made simple with the Quant Calibration application in the Resolutions Pro software.

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