Agilent Multiple Affinity Removal Spin Cartridge Quick Reference Guide

About this manual

This guide provides step-by-step instructions for preparing, washing, and eluting samples using the Agilent Multiple Affinity Removal Spin Cartridge. It details dilution, filtration, buffer exchange, and fraction collection procedures.

The guide also includes information on re-equilibration, storage, and analysis of collected fractions, with references to the full instruction guide for more details.

What's inside

• Dilute and filter sample
• Prepare spin cartridge
• Wash and collect flow-through F1
• Wash and collect flow-through F2
• Prepare for elution
• Elute bound fraction
• Re-equilibration
• Analyze F1 + F2

Frequently asked questions

What is the part number for the Agilent Multiple Affinity Removal Spin Cartridge?

The part number is 5188-5230.

What buffer is used to dilute the human serum sample?

Buffer A is used to dilute the human serum sample.

What is the centrifuge speed and time for collecting flow-through fraction F1?

Centrifuge at 100 x g for 1.6 min.

What buffer is used to re-equilibrate the cartridge after elution?

Buffer A is used to re-equilibrate the cartridge.