The document describes a method for protein separation and purification. The sample is diluted and filtered, and then applied to a spin cartridge for washing and collection. Finally, the bound proteins are eluted and the cartridge is re-equilibrated. The method is suitable for human plasma samples.
This guide outlines steps to dilute, filter, wash, and collect flow-through fractions from human plasma using the Agilent Plasma 7 spin cartridge. It includes procedures for re-equilibration and elution of bound proteins. Fractions F1 and F2 can be analyzed individually or combined for low-abundance proteins.
The guide references the full instruction manual for more details and accessories, and directs users to Agilent’s bioreagents website for related products.
Dilute 12–14 µL human plasma sample to 200 µL with Buffer A.
Yes, F1 and F2 can be analyzed individually or combined.