The document describes the operational steps for protein separation and elution, including dilution and filtration of the sample, preparation of a spin cartridge, application of the sample, washing and collecting the flow-through fractions F1 and F2, and elution of the bound fraction.
This guide provides step-by-step instructions for preparing, washing, and eluting samples using the Agilent Multiple Affinity Removal Spin Cartridge. It covers dilution, filtration, buffer exchange, and fraction collection for proteomics research.
The cartridge is designed for human serum and includes procedures for re-equilibration and storage. It is intended as a quick reference, with detailed instructions available in the full instruction guide.
The part number is 5188-5230.
Buffer A is used to dilute the human serum sample.
Centrifuge at 100 x g for 1.6 minutes.
Buffer A is used to re-equilibrate the cartridge.