This document describes the method and steps for using magnetic bead affinity chromatography to separate immunoglobulins, including diluting and filtering the sample, preparing the magnetic bead chromatography column, applying the sample, washing and collecting the flow-through F1, washing and collecting the flow-through F2, preparing for elution, eluting the bound fraction, re-equilibrating, and analyzing F1 + F2.
This guide provides step-by-step instructions for preparing, washing, and eluting proteins from the Agilent Multiple Affinity Removal Spin Cartridge using mouse serum. It includes dilution, centrifugation, buffer exchange, and fraction collection steps. The cartridge is designed for proteomics research and can be re-equilibrated for reuse. Additional products and resources are listed for further support.
The part number is 5188-5289.
Dilute 25-30-μL mouse serum sample to 200 μL with Buffer A.
Centrifuge 1.5 min at 100 x g.
Fill a new 5-mL plastic syringe with 4-mL Buffer A.